Cytoprotective Activities of Water-Soluble Fullerenes in Zebrafish Models - Zebrafish: Methods for Assessing Drug Safety and Toxicity - page 264

Table 19.4 Protection Provided by Fullerenes 1-4 Against General CNS Apoptosis Induced
by 6-OHDA

Maximum protection
against CNS apoptosis

Maximum protection against
dopaminergic CNS apoptosis Compound

1 23% ND

2 47% 100%

3 ND 60%

4 42% 60%

Table 19.5 Protection Provided by 4 Against Dopaminergic CNS Apoptosis Induced
by 6-OHDA

Treatment Animal 1 Animal 2 Animal 3 Animal 4 Animal 5

Control 28 29 30 26 32

6-OHDA 7 8 10 1 3

6-OHDAþ250 mM 4 22 14 21 18 26

Figure 19.7 Protection of CNS dopaminergic neurons from6-OHDA-induced apoptosis by 4. Untreated
(top panels), 6-OHDA plus vehicle treatment (middle panels), and 6-OHDA plus 250 mM 4 (lower panels)
embryos were stained with anti-tyrosine hydroxylase antibody. Five images of each group were examined;
the number of TH-immunoreactive cells clustered in diencephalons (between the eyes) was examined.
In untreated animals, 20-40 TH cells were observed (dark gray arrows). After 6-OHDA treatment, decrease
in the number of TH-immunoreactive cells was observed (1-17) (light gray arrows). After 250 mM
4 treatment, three of the five 4-treated animals showed a normal number of TH-immunoreactive cells with
normal morphology (black arrows).