Microfluidic Tools for DNA Analysis - DNA Nanotechnology: From Structure to Function - page 121

Fig. 7.7 Dam chip used in solid-phase rolling circle amplification ( RCA ). ( a ) Basic principle of
on-bead RCA; ( b ) fluidic control and working principle of the microfluidic chip integrated with a
dam structure (Reproduced from Ref. [ 37 ] with permission of The Royal Society of Chemistry)

and increase the sensitivity (Fig. 7.7 b). The padlock probe was circularized by
the target DNA and hybridized the primer attached on the beads to initiate the
elongation (Fig. 7.7 a).

7.2.2 Sample-In-Answer-Out System

One of the goals of TAS is to miniaturize every part of the sample analysis process,

including sample pretreatment, extraction, separation, analysis, and readout. With
the development of fabrication technologies in micro- and nanoscale, microfluidic
chips could be fabricated with sufficient complexity so that they can integrate orig-
inally separate units, even could realize some operations traditionally considered
impossible.