Microfluidic Tools for DNA Analysis - DNA Nanotechnology: From Structure to Function - page 120

Fig. 7.5 (continued)

Fig. 7.6 On-chip solid-phase multiplexed strand displacement amplification ( SDA )( Left and
middle , reprinted with permission from Ref. [ 35 ] Copyright (1997) National Academy of Sciences,
USA. Right , reprinted with permission from Macmillan Publishers Ltd: Ref. [ 34 ], copyright 2000)

targets within 1 mm 2 chip (Fig. 7.6 )[ 34 ]. The efficiency of the attachment of primers
and the specificity of the hybridization of primers with targets were enhanced by
the electric field which can be easily turned on and reversed [ 35 ]. In addition to
the flat surface amplification, SPA can also be carried out on beads. Recently,
Kitamori and coworkers developed an on-bead rolling circle amplification (RCA)
(Fig. 7.7 a), an isothermal amplification [ 36 ], which integrated a dam structure inside
the microfluidic channel for bead retention and enrichment to simplify the washing