Biomedical Engineering Reference
In-Depth Information
Figure 4.10
Chemical
structures of quaternary
ammonium salt lipids II.
CH
3
H
3
C
N
+
CH
3
N
+
H
3
C
CH
3
N
+
CH
3
CH
3
N
+
CH
3
H
3
C
CH
3
(
N
,
N
′
,
N
′′,
N
′′′
-hexamethyl,
N
,
N
′
,
N
′′,
N
′′′
-tetrapalmyl) spermine
H
3
C
N
CH
3
H
3
C
N
+
CH
3
N
+
CH
3
CH
3
N
CH
3
H
3
C
(
N
,
N
′
,
N
′′,
N
′′′
-tetramethyl,
N
,
N
′
,
N
′′,
N
′′′
-tetrapalmyl) spermine
with DOPE (
Fig. 4.10
)
[205]
. These compounds demonstrated comparable transfec-
tion efficiency with reduced cytotoxicity. University of California described a family
related to DOTAP/DOTMA, possessing two quaternary ammonium salts, for example,
PolyGum/DOPE (
Fig. 4.11
)
[206]
. These compounds demonstrated enhanced
in vitro
gene transfer on NIH3T3 cells as compared to DOTAP/DOPE. Imar Pharmaceutical
Corp. presented quaternary ammonium salt
N
,
N
-bis(hexadecyl aminocarbonyl meth-
ylene)-
N
,
N
-bis-(trimethyl ammoniummethyl aminocarbonyl methylene) ethylene
diamine diiodide and derivatives that demonstrated enhanced transfection activity
in
vitro
as compared to Lipofectin
TM
or DOTAP
[207]
.
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