Biomedical Engineering Reference
In-Depth Information
Fig. 4
Analysis of the biogenesis of LDs in HepG2 cells. HepG2 cells were transfected with GFP-Rab40c and
induced by oleic acid 0, 12, 24, and 48 h, respectively. Rab40c was revealed by GFP and LDs were revealed
by Oil Red under confocal microscopy. The results showed that GFP-Rab40c is recruited to LDs upon stimula-
tion in a time course dependent manner. Bar = 20
ΚΌ
m. This fi gure is reproduced from the open access journal
PLoS One [
19
]
because oleic acid is viscous. For complexing oleic acid and
BSA, oleic acid and BSA were mixed by gentle inversion and
swirling to avoid excessive foaming in a 50 ml tube until the
solution becomes clear.
