Biomedical Engineering Reference
In-Depth Information
a
Cells
Oil
b
c
d
e
Gd V~
Live
Dead
Oil
488 nm 555 nm
Electro-
fusion
Mixing
module
Delay line
(~15 min incubation)
Detection
module
Dyes
a
Oil
50 µm
b
d
c
Cells
Laser slit
Dyes
e
100
80
60
f
g
600
40
20
0
400
24
48
72
96
Incubation time (h)
0 h
24 h
48 h
72 h
96 h
200
0
1
2
3
4
5
6
7
8
Number of cells per droplet
FIGURE 5.33
High.cellular.viability.in.microdroplets..(From.Eric.Brouzes,.Martina.Medkova,.Neal.
Savenelli,.Dave.Marran,.Mariusz.Twardowski,.J..Brian.Hutchison,.Jonathan.M..Rothberg,.Darren.R..
Link,.Norbert.Perrimon,.and.Michael.L..Samuels,.“Droplet.microluidic.technology.for.single-cell.
high-throughput.screening,”.
Proc. Natl. Acad. Sci. U. S. A.
.106,.14195,.2009..Figure.contributed.
by.Eric.Brouzes.)
reported a high-throughput cytotoxicity screening system capable of encapsulating human
monocytic U937 cells in oil droplets over a period of approximately 4 days—a surprisingly high
viability (
Figure 5.33
). David Weitz laboratory at Harvard University has also demonstrated
microluidic droplet systems capable of encapsulating single cells in droplets and sorting the
droplets at high throughput based on luorescent indicators.
5.4 Microluidic Cell Culture Laboratories
his section reviews the technologies that have been developed for culturing cells in microlu-
idic environments, mostly geared toward cell biology and tissue engineering research, but also
toward stem cell biotechnology and high-throughput pharmaceutical testing.
