Biomedical Engineering Reference
In-Depth Information
(a)
(b)
(c)
250 µm
(e)
(f )
(d)
(g)
50 µm
25 µm
25 µm
250 µm
(h)
250 µm
FIGURE 5.31
Microcontainers.loaded.with.biological.contents..(From.Timothy.G..Leong,.Christina.
L.. Randall,
Bryan. R.. Benson,. Aasiyeh. M.. Zarafshara. and. David. H.. Gracias,. “Self-loading. litho-
graphically.structured.microcontainers:.3D.patterned,.mobile.microwells,”.
Lab Chip
.8,.1621-1624,.
2008..Reproduced.with.permission.from.The.Royal.Society.of.Chemistry.)
Ethanol
(a)
(b)
Glass
capillary
Deposit
plugs for
FISH
PDMS membrane
Live
Fixed
Teflon
tubing
Teflon
tubing
bacteria
in media
bacteria in
50% ethanol
Glass slide
600
500
400
PDMS T-junction
10000
(c)
(d)
Blank (Pc196)
E. coli
(Pc196)
Pc
(Pc196)
8000
6000
0 10 20
4000
2000
0
Plug 3
Plug 4
Plug 6
Pc
E. coli
Blank
5
10
15
20
Plug 3
Plug 4
Plug 6
FISH
Distance (pixels)
(e)
Cultured
Pc
Glass slide
Live culture
Split into
daughter
arrays
1 mm
Plug 3
Plug 4
Plug 6
Plug 6
Culture plate
FIGURE 5.32
Bacterial.cell.cultures.and.assays.in.microdroplets..(From.Weishan.Liu,.Hyun.Jung.
Kim,.Elena.M..Lucchetta,.Wenbin.Du,.and.Rustem.F..Ismagilov,.“Isolation,.incubation,.and.parallel.
functional.testing.and.identiication.by.FISH.of.rare.microbial.single-copy.cells.from.multi-species.
mixtures.using.the.combination.of.chemistrode.and.stochastic.coninement,”.
Lab Chip
.9,.2153-
2162,.2009..Reproduced.with.permission.from.The.Royal.Society.of.Chemistry.)
