Biomedical Engineering Reference
In-Depth Information
1.2
1.0
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(a) Straight
1 3
5 7
9 11
(b) Fall
1.2
1.0
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9 11
(c) Rise
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Cavities
FIGURE 3.77
Gradient.generator.based.on.transport.through.microtunnels..Scale.bar.is.200 μm.
(From. Cheuk-Wing. Li,. Rongsheng. Chen,. and. Mengsu. Yang,. “Generation. of. linear. and. non-linear.
concentration.gradients.along.microluidic.channel.by.microtunnel.controlled.stepwise.addition.of.
sample. solution,”.
Lab Chip
. 7,. 1371-1373,. 2007.. Reproduced. with. permission. from. The. Royal.
Society.of.Chemistry.)
gradient; (3) downstream cells are exposed to higher concentrations of cell-secreted molecules
(e.g., metabolites and growth factors) than upstream cells, which precludes true redundancy in
single-cell data; and (4) the observation area is an enclosed microluidic channel, which limits
gas and nutrient exchange for long-term cell viability and hinders physical access to single cells
(e.g., patch-clamp recording, intracellular injection, atomic force microscopy, etc.) during the
(a)
(b)
Input
Input
Cell culture
channels
Collection
well
0.5 mm
5 mm
FIGURE 3.78
Linear. dilution. generator.. (From. Glenn. M.. Walker,. Nancy. Monteiro-Riviere,. Jillian.
Rouse,.and.Adrian.T..O'Neill,.“A.linear.dilution.microluidic.device.for.cytotoxicity.assays,”.
Lab Chip
.
7,.226-232,.2007..Reproduced.with.permission.from.The.Royal.Society.of.Chemistry.)
