Biomedical Engineering Reference
In-Depth Information
Device
Resistance model
Fluorescence measurements
a
Sample
1.0
Results
Theory
f(x)
= x
R
2
= 0.9983
n
= 7
0.8
Buffer
Scanning
direction
0.6
Single
module
0.4
0.2
Scanning
zone
#0 #1 #2 #3 #4 #5
Linear profile
0
#0
#1 #2 #3
Contact number
#4
#5
Outlet
b
Sample
1.0
0.8
0.6
Results
Theory
Buffer
Scanning
direction
f(x)
= log
2
x
R
2
= 0.9998
n
= 7
Single
module
0.4
0.2
Scanning
zone
#0 #1 #2 #3 #4 #5
0
#0
#1
#2
#3
#4
#5
Outlet
2-fold logarithmic
Outlet number
c
1.0
f(x)
= ce
(
_____
)
-
(x-a)
2
2
s
2
Results
Theory
Bu“er
Sample
0.8
0.6
A
c
=
Scanning
direction
R
2
= 0.9992
n
= 7
√2π
2
A
=0.8278
a
=1
s
=0.33
Module
B
Module
A
0.4
0.2
Scanning
zone
#1
#2
#3
#4
#5 #7
#9
#0
#6
#8 #10
Outlet
0
#0 #1 #2 #3 #4 #5 #6 #7 #8 #9#10
Gaussian
Outlet number
FIGURE 3.75
Microluidic. gradient. generators. for. arbitrary. gradients. using. electrical-circuit. ana-
logues.. (From. Kangsun. Lee,. Choong. Kim,. Byungwook. Ahn,. Rajagopal. Panchapakesan,. Anthony.
R. Full,.Ledum.Nordee,.Ji.Yoon.Kang,.and.Kwang.W..Oh,.“Generalized.serial.dilution.module.for.
monotonic.and.arbitrary.microluidic.gradient.generators,”.
Lab Chip
.9,.709-717,.2009..Reproduced.
with.permission.from.The.Royal.Society.of.Chemistry.)
9
Cultivation
chamber
8
a
b
c
Principle of operation
Detection point
7
b
1
:
a
1
Detection
point
Distribution
channels
Ch. 9
8
7
6
5
4
3
2
1
6
Inlet 1
a
1
b
1
5
b
2
:
a
2
Inlet 1
Inlet 2
a
2
b
2
4
Liquid A
Liquid B
b
3
:
a
3
Inlet 2
Inlet branch
channel
a
3
b
3
Confluent
points
3
2
10 mm
Outlet 1
500 µm
Distribution channel Confluent point
FIGURE 3.76
Dilution.generator.based.on.ratiometric.distribution.of.low.resistance..(From.Masumi.
Yamada,.Takaya.Hirano,.Masahiro.Yasuda,.and.Minoru.Seki,.“A.microluidic.low.distributor.generat-
ing.stepwise.concentrations.for.high-throughput.biochemical.processing,”.
Lab Chip
.6,.179,.2006..
Reproduced.with.permission.from.The.Royal.Society.of.Chemistry.)
