Chemistry Reference
In-Depth Information
Tropocollagen
α
1
α
1
α
2
Telopeptide
(non-helical region)
Telopeptide
(non-helical region)
Part of collagen fibril
0.5 M acetic acid
4°C
Pepsin
Pepsin-solubilized collagen
Dialyzable peptides
Fig. 10.2
Role of pepsin in the cleavage of telopeptide of collagen fibrils.
proteases, but the process takes an extended time. Fish protein hydrolyzate from Pacific hake
containing high endogenous proteolytic activity from
Kudoa paniformis
parasitic infection
could be prepared by autolysis at 52
◦
CatpH5.5for1h.
42
To expedite the hydrolysis pro-
cess, commercial proteases as well as other proteases have been used widely to hydrolyze
proteins, particularly those obtained as by-products or from underutilized species. Some
commercial proteases used for the production of fish protein hydrolyzate include Alcalase,
Neutrase, Flavourzyme, Protamex, pepsin, chymotrypsin, trypsin, papain, Pronase E, colla-
genase, etc.
43-51
(see Table 10.3).
In general, different proteases exhibited varying characteristics, including optimal pH
and temperatures. Therefore, to maximize hydrolysis, it should be conducted under the
appropriate conditions. Additionally, the degree of hydrolysis (DH) can be manoeuvred to
obtain a hydrolyzate with desirable properties. Once the required DH is obtained for the
hydrolysis, inactivation of protease is required. Otherwise, the DH cannot be controlled
and subsequent undesirable effects, particularly the formation of bitterness or the loss in
