Chemistry Reference
In-Depth Information
Table 10.2
Pepsins used for collagen extraction.
Concentration
of
Digestion
ASC yield
PSC yield
Raw materials
Pepsin
pepsin used
time
(%)
(%)
References
10 U g
−1
Threadfin bream
skin
Albacore
tuna
skin
12 h
22.45
74.48
35
Skipjack
63.81
Tongol
71.95
Porcine
75.92
Yellowfin tuna
dorsal skin
ND
0.98% (w/v)
23.5 h
-
27.1
36
20 kUnits g
−1
skin
Bigeye snapper
skin
Bigeye
snapper
48 h
5.31
18.7
30
Grass carp skin
Porcine
1% (w/v)
24 h
-
46.6
34
Black drum skin
ND
0.1% (w/v)
3 days
2.3
15.8
37
Sheephead
seabream skin
ND
0.1% (w/v)
3 days
2.6
29.3
Brownstripe red
snapper skin
Porcine
10% (w/v)
48 h
9
4.7
27
Deep-sea redfish
skin
Porcine
0.1% (w/v)
48 h
47.5
92.2
38
Sardine scale
Porcine
10% (w/w)
24 h
-
50.9
Red sea bream
scale
Porcine
10% (w/w)
24 h
-
37.5
28
Japanese sea
bass scale
Porcine
10% (w/w)
24 h
-
41
Grass carp scale
ND
1% (w/v)
48 h
-
25.64
39
Bullfrog skin
ND
0.1 g/40 g skin
8 h
-
12.6
40
Octopus Callistopus
arakawai arm
Porcine
10% (w/v)
48 h
10.4
62.9
31
Cuttlefish outer
skin
Porcine
10% (w/v)
48 h
2
35
33
Paper nautilus
outer skin
Porcine
10% (w/v)
2 days
5.2
50
32
Rhizostomous
jellyfish mesogloea
Porcine
10% (w/v)
48 h
-
35.2
41
α
β
However, degradation of
components took place when pepsin from skipjack tuna
was used (see Fig. 10.3). Albacore pepsin showed comparable extraction efficiency to porcine
pepsin and had no adverse effect on the integrity of resulting collagen. Thus, albacore pepsin
could be used for collagen extraction from threadfin bream skin.
35
and
10.2.1.6
Protein hydrolyzate
Fish protein hydrolyzate can be prepared with the addition of protease to increase the endoge-
nous hydrolytic activity and shorten the hydrolysis time. Enzymatic hydrolysis possesses a
greater advantage when compared with chemical hydrolysis or autolysis caused by the
endogenous enzymes in fish. Hydrolyzates can be obtained by maximization of endogenous
