ENGINEERING OF CELL-PENETRATING PEPTIDE-CONJUGATED INTRACELLULAR DELIVERY SYSTEMS - Biologically-Responsive Hybrid Biomaterials - page 222

heterobifunctional PEG spacer resulting in a TAT-PEG-PEI conjugate. This was
used to complex DNA and significantly higher transfection efficiencies for
TAT-PEG-PEI were detected in mice [130] due to the stabilizing effect of PEG
in vivo .
Suk et al. attached RGD or HIV-1 TAT peptides to PEI/DNA
nanocomplexes via PEG spacer molecules. Both RGD and TAT improved the
cellular uptake of gene vectors and enhanced gene transfection efficiency of
primary neurons up to 14-fold [49].
Use of lysomotropic agents such as chloroquine has been used in
combination with PNA for efficient splice correction [45, 50, 51]. However this
has no application in vivo due to its pharmacological effects.
In any case, it looks like CPPs are really becoming promising tools for the
intracellular delivery of various drugs and drug-loaded pharmaceutical

nanocarriers.

List of Acronyms and Abbreviations

Cell-penetrating peptides CPPs

Protein transduction domain PTDs

Membrane translocating sequences MTSs

Model amphipathic peptide MAP

Human immunodeficiency virus type 1 HIV-1

poly(2-hydroxytethyl aspartamide) PHEA

Antibody targeted, triggered, electrically
modified prodrug-type strategy

ATTEMPTS

Asparaginase ASNase

Tissue-specific plasminogen activator t-PA

Elastin-like polypeptide ELP

Cytotoxic T-lymphocyte CTL

Dendritic cell DC

Heat shock protein 70 HSP70

Magnetic resonance imaging MRI

Shell cross-linked SCK

FITC-doped silica nanoparticles FSNPs

Bovine serum albumin BSA

Quantum dots QDs