Biology Reference
In-Depth Information
Table 6. Theoretical Conversion Table from Stoichiometry to Slopes
Slopes
Slopes
Slopes
Stoichiometry
(tan)
Stoichiometry
(tan)
Stoichiometry
(tan)
1
1
9
4.33
17
7.72
2
1.48
10
4.80
18
8.14
3
2.05
11
5.22
19
8.56
4
2.31
12
5.67
20
8.98
5
2.73
13
6.05
21
9.40
6
3.14
14
6.47
22
9.82
7
3.56
15
6.89
23
10.24
8
3.97
16
7.31
24
10.66
(Adapted from Ref. 151, with permission from the author and the publisher, Elsevier.)
already a published predicted secondary structure of the pRNA,
which revealed two loops, termed the left- and right-hand loops.
121
The sequences of these two loops (specifically, bases 45-48 of the
right-hand loop and bases 82-85 of the left-hand loop) are naturally
complementary and were originally proposed to form a pseudo-
knot.
127
Further studies revealed that these two sequences interact
intermolecularly, allowing the formation of pRNA oligomers. A vari-
ety of mutated left- and right-hand loop sequences for several pRNA
were constructed. In the developed notation, the same letter in
upper- and lower-case represented a pair of complementary
sequences. For instance, in pRNA
A-a´
, the right and left loops are
complementary, while for pRNA
A-b
´, the four bases in the right loop
A are not complementary to the left loop
b
´. Those pRNAs with com-
plementary loop sequences are active in DNA packaging, while those
mutants with non-complementary loops are inactive.
Three sets of pRNAs were produced in order to facilitate this
process.
One Set Composed of Two Engineered pRNAs:
Multiple of 2
A key finding in pRNA research was that the mixing of two mutant
pRNAs with trans-complementary loops restored DNA packaging
