Biology Reference
In-Depth Information
Fig. 12.
Plotting of stoichiometry against slopes of dose-response log/log
plot curves of components in phi29 assembly
in vitro
. The equations were
derived from the best-fit curve with an
R
square of 0.998. (Adapted from
Ref. 151, with permission from the author and the publisher, Elsevier.)
Common Multiples
In order to eliminate certain possible stoichiometric values for bio-
logical assemblages, attempts have been made to quantify which com-
binations of building blocks come together to form a larger unit, thus
suggesting that these subunits make up a constituent part of the sys-
tem as a whole in question. The usual process involves the manipula-
tion of the building blocks (each of which are necessary for proper
functionality of the larger subunit under investigation) to allow for
complementarity, and subsequent testing to reveal the extent to
which these altered building blocks cooperate with one another to
form a larger subunit. A recent example of this is the study conducted
on pRNA, in which two inactive mutant pRNAs with complementary
loops were mixed intermolecularly and then assayed for the relative
amount of activity exhibited in DNA packaging.
133,137
There was
