Biology Reference
In-Depth Information
Fig. 6.
Steps in the synthesis of infectious virions of pho29
in vitro
.
(Adapted from Ref. 31, with permission from the author and the publisher,
American Society for Microbiology.)
Fig. 7.
Assembly pathway of phi29
in vitro
.
packaging is three, the probability of all combinations of mutant and
wild-type pRNAs on a given procapsid can be determined by the
expansion of the binomial: (
p
100%.
That is, in the procapsid population, the probability of procapsid pos-
sessing either three copies of mutant pRNA, two copies of mutant
and one copy of wild type, one copy of mutant and two copies of wild
type, or three copies of wild type is
p
3
, 3
p
2
q
,
+
q
)
3
=
p
3
+
3
p
2
q
+
3
pq
2
+
q
3
=
+
3
pq
2
, or
q
3
, respectively.
Suppose that there were 70% (
p
100%)
wild-type pRNA in the reaction mixture, then, the percentage of pro-
capsids that possessed at least two copies of wild-type pRNA would
×
100%) mutant and 30% (
q
×
