Biology Reference
In-Depth Information
Figure 11:
Schematic representation of the syntenic regions within the toxin gene clusters in
C. raciborskii
CS-505 and
R. brookii
D9. (A). Location of the CYN gene cluster of
C
.
raciborskii
CS-505 compared with the syntenic genomic region in
R. brookii
D9.
(B). Gel electrophoresis of the PCR products from the
hypF/hupC
amplifi cation in
R. brookii
D9 and in the strains of
C. raciborskii
non-toxic: CS-507, CS-508, CS-509 and CS-510. Producers of CYN: CS-505, CS-506 and CS-511 do not present amplifi cation of
the
hypF/hupC
region. C. Location of the STX gene cluster of
R
.
brookii
D9 compared with the syntenic genomic region in
C
.
raciborskii
CS-505. Genes participating in syntenic regions are depicted in blue and highlighted in the green boxes within the
arrows; genes outside the syntenic regions are depicted in white. tRNAs and transposases are shown in red. The grey arrows
show the position of the primer pairs HYPa/HUPa and HYPb/HUPb used to amplify the region between
hypF
and
hupC
genes in different strains of
C. raciborskii
and in
R. brookii
D9, respectively. Ladder: GeneRuler 1 kb DNA ladder (Fermentas,
Ontario, Canada). With the kind permission of Moníca Vásquez, Department of Molecular Genetics and Microbiology, Faculty
of Biological Sciences, Pontifi cia Universidad Católica de Chile, Santiago, Chile & Millenium Nucleus EMBA, Santiago, Chile
[Stucken
et al
. (2010)
PLoS ONE
5(2):
e9235; doi:10.1371/journal.pone.0009235] doi:10.1371/journal.pone.0009235.g004.
Color image of this figure appears in the color plate section at the end of the topic.
