Simultaneous Determination of Caffeine and Phenolic Compounds in Tea and Coffee - Caffeine: Chemistry, Analysis, Function and Effects - page 141

Table 8.3 (Continued)

Sample Target analytes Chromatographic conditions Elution Detection Ref

Coffee
(instant)

Solvent: water (1% phosphoric acid)
(solvent A) and acetonitrile (1%
phosphoric acid) (solvent B)

0 min, 5% B; 0.5 min,
10% B;

Fluorescence

Column: Kinetex C18, (2.6mm, 100
6 4.6mm)

2.0 min, 12.5% B; 3.0
min, 15% B; 4.0 min,
80% B; 5min, 100% B;
6.0 min, 100% B; 7.0
min, 5% B

UV: 270nm,
280 and
320nm
Fluorescence

Temperature: 55 0 C
Flow rate: 2.2 mL min -1
Run time: kaempferol-3- O-
glucoside, 4.66 min
Equilibration time: 3 min

Tea Esculin, Protocatechuic acid,

(GA), (PCCA), (CHLA),
(VA), (CA), (SA),(FA),
(CNA), (C), (CG), (EC),
(ECG), (EGC), (EGCG),
(GC), (GCG), (RUT),
(MYR), (QCI), (QCE),
(NAR), (LUT), (HES),
(KAEM), (AES), (SCOP),
(4OH-COUM), (6M-
COUM), (O-CMA)
and(CAF)

Injecton Volume: 2 mL Gradient: UV: 280nm Nov´ kov´

et al 2010 Solvent: 0.1% formic acid (solvent 0-4 min, (88.5% A :

11.5% B);

A) and methanol (solvent B) 4-20 min, linear gradient

elution from 88.5% A :
11.5% B to 50% A :
50% B.

Column: BEH C18 (100 mm 6 2.1
mm, 1.7 mm)
Temperature: 25 0 C
Flow rate: initial 0.45mL min -1
Run time: Kaempferol, approx. 18
min
Equilibration time: n. e.