Simultaneous Determination of Caffeine and Phenolic Compounds in Tea and Coffee - Caffeine: Chemistry, Analysis, Function and Effects - page 140

Table 8.3 Survey of methods for the simultaneous analysis of alkaloids and polyphenols from tea and coffee.

Sample Target analytes Chromatographic conditions Elution Detection Ref

Tea
(Oolong,
Pu-erh,
green,
black)

(GA), (3,5-HBA), (3,4,5-
MCNA), (MYR), (CAF),
(p-ASA), (CG),
(EGC),(EGCG) and (ECG)

Injection volume: 10 uL Gradient: UV: 280 nm Zuo et al

2002 Solvent: water-acetic acid, (97 : 3 v/

v) (solvent A); methanol (solvent
B)

0-1 min, 0% B; 1-27min,
0-63% B.

Column: Alltech Adsorbosil C18
(250 mm x 4.5 mm, 5 mm)
Temperature: n. e.
Flow rate: 1 mL min -1
Run time: 3,4,5-trimethoxycinnamic
acid, approx. 25 min
Equilibration time: 2 min

Tea
(black,
white,
green)

(CAF), (GA), (PCCA),
(CHLA), (CA), (p-CMA),
(GCG), (EC), (C), (EGC),
(GC), (EGCG), (ECG),
(MYR3RM), (QCE3R),
(QCE3GA), (KAEM3R),
(KAEM3G), (AP-7-O-G),
(Lut-7-O-G)

Injection volume: 10 mL Gradient UV: 270nm,

280 and
320nm

Rostagno
et al 2011