Differentiation in Gonochores - Genetic Sex Differentiation in Fish - page 44

Table 14. List and functions of mutant XY female from wild medaka populations of Japan
(compiled from Otake et al., 2006, with permission by the Genetic Society of America)

Site Prefecture Genotype Phenotype

sex of XY m F 1

Remarks

Aomori Aomori XY m all ♀♀

Aizu-bange Fukushinia XY m all ♀♀

Kurobe Toyama XY m all ♀♀ Mutant DMY gene is not

functional

Suzu Ishikawa XY m all ♀♀

Awara Fukui XY m all ♀♀

Aki Kochi XY m all ♀♀

Oura Kagoshima XY wOur all ♀♀ Lost ♂ determining function

Saigo Shimane XY mSai all ♀♀

Kesen-numa Miyagi XY wKsn ♀♀ + ♂♂ Two XY wKsn /XY m

are required to determine
♂ sex

Auza-Wakamatsu Fukushima XY m ♀♀ + ♂♂

Shirone Niigata XY m ♀♀ + ♂♂

Fig. 11. Nucleotide sequences sex reversed mutant and medaka Oryzias latipes . The poly C
tract specifi c to northern populations of HNI, Awara and Suzu are underlined. The Awara
XY ♀ has a cytosine insertion at amino acid 110, the Suzu XY♀ cytosine deletion at amino
acid 109 and the Aki XY ♀ cytosine insertion at amino acid 102, each of which results in a
frameshift and premature termination. The nucleotide sequences of Hd-rRY HNI, and carbio
XY females are mutants at the poly C tract in exon 3 (from Otake et al., 2006, with permission
by the Genetic Society of America)

with normal DMY coding sequence (Fig. 11). The mutations included (i) a
C insertion in a poly C tract and deletions in exon 3, (ii) a C deletion in the
same poly C tract in exon 3, (iii) a C deletion in another site in exon 3 and
(iv) a large insertion or deletion in intron 5 and/or exon 6 in the Saigo strain
(Y mSai Y mSai ) XY females and possess no polyadenylation signal.