Digital Optical and Scanning Probe Microscopy for Inspection and Manipulation of Biocells - Bioanalysis Advanced Materials Methods and Devices - page 91

Fig. 7.3 AFM image of erythrocytes. On the left top-down : normal cell, scanning area
12.0 12.0 m m; erythrocyte after load increase (115 nN), scanning area 12.0 12.0 m m; scratch
on the cell's surface, scanning area 1.4 3.4 m m. On the right top-down : normal cell, scanning
area 10.0 10.0 m m; erythrocyte after load increase (160 nN), scanning area 10.0 10.0 m m;
hollowing, scanning area 3.0 1.9 m m
Using the higher force, then, means the cell may be cut sufficiently strongly to
enable the (inner) contents to be investigated.
Also, we calculated the value of the force at the four load parameters. There
was a linear dependence between the applied load and the depth of indentation.
But the values of maximum forces differed and the range of penetration depths
was greater with increasing force and with damage. This is shown in Fig. 7.4