Applications of Mass Spectrometry in Analyses of Steroid Hormones - LC-MS in Drug Bioanalysis - page 254

Table 1 Representative reviews on steroids analyses by LC-MS/MS and GC-MS
Steroid analysis Major topics Reference

Steroid hormones in clinical
chemistry

Role of MS in clinical diagnostic testing and
endocrine biomarker analysis; sample
preparation, e.g., LLE vs. SPE, and
derivatization to enhance sensitivity; LC-MS/
MS method validation, specificity, quality
management, interpretation of test results for
clinical diagnosis; LC-MS/MS analysis of
steroid hormones and related physiology/
pathology diagnostic significances,
including: adrenal steroids, glucocorticoids,
androgens and estrogens.

[ 2 ]

Steroids hormones in clinical
chemistry

Comparison of RIA, GC-MS/MS and LC-MS/
MS in steroid analysis; role of LC-MS/MS in
metabolomics, e.g., quantitative bioanalysis,
identifying and profiling biomarkers;
standardization of MS assays.

[ 14 ]

Steroids hormones in clinical
chemistry

Steroid analysis by LC-MS/MS in pediatrics—
challenges in method specificity, sensitivity,
and test results interpretation; steroid assays
and profiling in plasma, saliva, and urine by
LC-MS/MS.

[ 9 ]

Steroids hormones in clinical
chemistry

Immunoassay vs. GC-MS and LC-MS/MS;
derivatization vs. nonderivatization; steroid
profiles for newborns, adrenal insufficiency,
prostatitis/pelvic pain syndrome, premature
adrenarche, sera from smokers, metabolic
diseases, diabetes, water contaminant,
athletes doping.

[ 3 ]

Estrogens in clinical chemistry RIA, GC-MS/MS, and LC-MS/MS analyses of

estrogens in serum and plasma; isotope
internal standard; sample derivatization;
ionization modes and sensitivities of
GC-MS/MS and LC-MS/MS.

[ 4 ]

Estrogens in clinical chemistry Measurement of endogenous estrogens by

immunoassays, LC-MS (ESI, APCI, APPI)
and GC-MS.

[ 15 ]

Endogenous conjugated
androgens in clinical
chemistry

GC-GS and LC-MS/MS analyses of conjugated
androgens—deconjugation, derivatization,
and associated issues.

[ 7 ]

Steroids hormone residuals in
meat safety concerns

GC-MS n and LC-MS n analyses of steroids in
edible matrices, e.g., meat, liver, kidney,
kidney fat, and milk.

[ 12 ]

Steroids hormones as
environmental pollutants

Analysis of steroids as environmental endocrine
disrupting compounds; sample preparation,
e.g., LLE vs. SPE; immunoassay vs. GC-MS/
MS and LC-MS/MS analyses; sensitivities,
e.g., LOD at pg-ng/mL level.

[ 13 ]

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