Biomedical Engineering Reference
In-Depth Information
Table 2
Overview of
selected TDM methods
Author
Matrix
Sample [mL]
Drugs
IS
“Work Up”
Stationary phase
Mobile phase
Detection mode
Validation data
Ref.
Berna et al.
Plasma
+ serum
0.5
Olanzapine,
N
-desmethyl-
olanzapine,
2-hydroxymethyl-
olanzapine,
olanzapine-
N
-
oxide,
olanzapine-10-
N
-
glucuronide
LY017222 (structural
analog)
96-well SPE
MetaChem
monochrom
HPLC
(4.6 × 150 mm,
5 m m)
Gradient with
10 mM
ammonium
acetate and
propane-1-ol
in MeOH
APCI, positive
mode, MRM,
MS/MS
Linearity, LLOQ,
accuracy,
precision,
extraction
ef fi ciency,
matrix effects,
selectivity, F/T
and LT
stability,
processed
sample
stability
[
45
]
Cabovska
et al.
Plasma
0.2
Risperidone,
9-OH-risperidone
R068808 (structural
analog),
(+)9-OH-risperidone;
(-)9-OH-risperidone
LLE (MTBE)
Chiralcel OJ column
(50 mm × 4.6)
10 mM ammonium
acetate in 50:50
EtOH/PrOH
(v/v) and hexane
ESI, positive mode,
MRM, MS/MS
Matrix effects,
precision,
accuracy,
selectivity,
recovery, ion
suppression,
processed
sample
stability
[
53
]
Chew et al.
Plasma
0.5
Buspirone
Prazosin
SPE
Luna C18
(50 mm × 2.5 m m)
Gradient with
TFA in dH
2
O
and TFA in
ACN
ESI, positive mode,
MRM, MS/MS
LLOQ, accuracy,
precision,
extraction
recovery,
matrix effects
[
62
]
Gschwend
et al.
Plasma
0.25
Amisulpride
Sulpiride
LLE (diisopropylether:
dichloromethane,
1:1)
Phenomenex
Synergi Polar-RP
analytical column
(75 mm
×
4.6 mm,
4 m m)
Isocratic with 5 mM
ammonium
formate/ACN
ESI, positive mode,
MRM, MS/MS
Linearity,
selectivity,
recovery,
precision,
accuracy, F/T
and LT
stability,
processed
sample
stability
[
63
]
