Biomedical Engineering Reference
In-Depth Information
A
scabies protein
hyman lgG
pre-immune
schematic
SMIPP-S
B2
Sar s 3
C1q
B
Sar S 3
SMIPP-S I1
Figure 4.3
(A) Immunohistochemical staining demonstrating gut localization of
SMIPP-Ss, Sar s 3, and complement. In consecutive human scabies mite
infested skin sections antibodies against a representative SMIPP-S, Sar s 3
and co-localized with anti-human IgG (used as a marker to label the gut).
(B) Mite gut molecules, detected in the mite feces within the burrows
(bottom row). Figure modified from various sources.
43,44,60
functions. Analysis of the predicted molecular surface properties of all 33
SMIPP-Ss identified regions of conservation in other regions on the surface of
the molecule that could present alternative sites for interaction.
59
4.4.1 Scabies Mite Inactive Protease Paralogues Inhibit
Complement
Functional studies of two SMIPP-Ss I1 and D1 selected from different clades in
the phylogenetic tree (Figure 4.1) identified them as potent inhibitors of the
complement system.
43
Both SMIPP-Ss inhibit the haemolytic activity of human
serum by binding to the complement factors C1q, Mannose Binding Lectin
(MBL) and properdin (Figure 4.4). We found that the SMIPP-Ss interact
mainly with the collagenous stalk of C1q (Figure 4.4). These three complement
factors affected are essential for the activation of the three pathways of the
