Biomedical Engineering Reference
In-Depth Information
1.
2.
3.
4.
5.
6.
8.
7.
FIGURE 11.25
Schematic illustration of sandwich ELISA mode. Adapted from http://pl.wikipedia.
org/wiki/ELISA
there still exist considerable obstacles associated with commercialization. Attachment
of the bait protein to the array surface can be accomplished through either non-specifi c
adsorption or through direct covalent binding. Critical limitations include protein dena-
turation upon interaction with the surface, disruption of the interaction domain through
poor orientation or steric hindrance caused by physical closeness to the support itself
(particularly important with small bait molecules), or a lack of suffi cient density to
allow for subsequent detection. Additionally, the range of biologically signifi cant affi ni-
ties (mM to pM) that must be considered when optimizing target binding conditions to
Search WWH ::
Custom Search