Biomedical Engineering Reference
In-Depth Information
A
60
45
30
15
2.5
5.0
7.5
10.0
c
/ mM
60
B
50
40
30
20
10
0
0.2
0.4
0.6
0.8
1.0
-1
/ mM
-1
c
Figure 14. A) Conductance change ǻ
G
of a gramicidin D-
doped pore-spanning membrane in the absence (filled sym-
bols) and presence of 20 mM CaCl
2
(open symbols). LiCl
(Ŷ), LiCl + 20 mM CaCl
2
(Ƒ), NaCl (Ɣ), KCl (Ÿ), KCl + 20
mM CaCl
2
(ǻ). The solid lines are linear regressions yielding
slopes for the mean conductance per concentration unit in the
absence of Ca
2+
.
m
Li
= (1.29 ± 0.05) μS mM
-1
,
m
Na
= (3.3 ±
0.2) μS mM
-1
,
m
K
= (6.3 ± 0.3) μS mM
-1
. The dashed lines
are linear regressions in the presence of CaCl
2
with the fol-
lowing slopes:
m
Li,Ca
= (0.585 ± 0.008) μS mM
-1
and
m
K,Ca
=
(2.76 ± 0.09) μS mM
-1
. Buffer: 10 mM TRIS, 100 mM TMA,
pH 8.6.
38
B)
Concentration resistance
ı
W
as a function of
c
-1
in the presence of (Ŷ) LiCl, (Ɣ) NaCl und (Ÿ) KCl. Linear
regressions result in the slope
s
and the intercept
ı
W,0
.
s
Li
=
44.2 kȍ s
-0.5
mM,
ı
W,0,Li
= 7.4 kȍ s
-0.5
,
s
Na
= 32.8 kȍ s
-0.5
mM,
ı
W,0,Na
= 6.6 kȍ s
-0.5
,
s
K
= 29.2 kȍ s
-0.5
mM,
ı
W,0,K
= 6.4 kȍ s
-
0.5
. Buffer: 10 mM TRIS, 100 mM TMA, pH 8.6.
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