Biomedical Engineering Reference
In-Depth Information
10
6
A
10
5
t
10
4
-75
-50
t
-25
0
10
-1
10
0
10
1
10
2
10
3
10
4
10
5
10
6
f
/ Hz
B
R
m2
hemifused
liposome
C
m2
cis
C
m1
R
m1
R
el
trans
C
C1
C2
R
el
R
el
R
m1
R
m2
R
m1
C
m1
C
m2
R
m2
C
m2
C
m1
Figure 5. A) Absolute value of the impedance |
Z
|(
f
) and
phase angle I(
f
) of a pore-spanning membrane on CPEO3-
functionalized porous alumina in the presence of a vesicle
suspension composed of DPhPC/DOPC 6:4 (Ŷ) 4 hours,
(Ƒ) 9 hours, (Ɣ) 12 hours, (ż) 15 hours and (Ÿ) 22 hours
after the addition of LUVs. The arrow indicates the time
course of the experiment. Of the two discernable disper-
sions at the beginning, only one dispersion is found at the
end of the incubation period. Electrolyte: 0.1 M NaCl.
35
B)
Scheme depicting the hypothesized intermediate state dur-
ing pore-spanning membrane formation, which leads to
two dispersions in the impedance spectra together with an
equivalent circuit representing the two membranes (
R
m1
,
C
m1
and
R
m2
,
C
m2
) and the electrolyte resistance
R
el
. C) Al-
ternative equivalent circuits to model the impedance data,
which feature two discernable dispersions with
R
m1
,
R
m2
,
C
m1
and
C
m2
and the electrolyte resistance
R
el
.
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