Biomedical Engineering Reference
In-Depth Information
TABLe 4.1
Applications.of.Biomolecular.Motors.in.Nanotechnology
Motor
Guidance
Experiment
Future Function/Application
Viability
Reference
Myosin
Glide;.applied.electric.
ield;.docked.motors.
on.PMMA.grating.or.
lat.sheet.of.myosin.
heads;.uniform.density
Inverted.motility.assay.using.
external.guidance.
mechanism;.actin-myosin
Nanoelectronic.device.
integration.(NEMS,.MEMS,.
lab-on-a-chip);.organization.
of.cargo-carrying.shuttles;.
self.sorting
Negatively.charged.tubulin.
subunits.orient.MTs.at.positive.
cathode;.several.disadvantages.
pose.threat.to.applicability.(see.
Section.4.4.4.in.text)
Riveline.et.al..
(1998)
Kinesin
Glide;.docked.motors.in.
polyurethane.tracks;.
uniform.density;.caged.
ATP.fuel.source
Inverted.motility.assay;.
kinesin-MT;.caged.ATP.for.
velocity.control.of.shuttles.
dictated.by.motor.domains
Control.of.molecular.shuttle.
motion.over.particular.
distances.on.inorganic.
devices
Caged.ATP.proven.a.feasible.
manner.in.which.to.manage.
molecular.shuttle.motility.and.
has.been.implemented.into.
attempts.at.practical.devices
Hess.et.al..
(2001);
Fischer.et.al..
(2009)
D. melanogaster
.
conventional.
kinesin.
(full-length.
expressed.in.
E. coli
.with.
HexaHis-tag)
Glide;.applied.magnetic.
ield.to.magnetically.
functionalized.MTs;.
docked.kinesin.
motors;.uniform.
density
Inverted.motility.assay.using.
external.guidance.
mechanism;.kinesin-MTs;.
CoFe
2
O
4
.nanoparticle.labels
Nanoelectronic.and.magnetic.
device.integration.(NEMS,.
MEMS,.lab-on-a-chip);.
organization.of.cargo-
carrying.shuttles;.self.sorting
Use.of.magnetically.distinct.
nanoparticles.properly.orients.
MT.molecular.shuttles;.may.
substitute.as.an.external.
guiding.device.over.electric.
ield.usage.or.function.in.
unison.with.the.latter;.not.
extensively.tested
Hutchins.et.al..
(2007)
Conventional.
kinesin
Bead.(kinesin.coated);.
parallel.and.isopolar.
MT.low.ield
Motility.assay;.kinesin-MT;.
glass,.gold,.and.polystyrene.
beads.transported.over.
2.2.mm;.90%.parallel.MT.
alignment.using.antibodies.
bound.to.α-tubulin
Transport.of.cargo.over.
signiicant.distances;.
transport.of.entire.
microchips
Classical.motility.assays.have.
not.been.recognized.as.
primary.candidates.for.device.
application;.mechanism.works.
experimentally;.dificult.to.
employ.practically
Bohm.et.al..
(2001);.
Limberis.and.
Stewert.
(2000)
E. coli
.
recombinant.
protein.
K560—dimeric.
H. sapien
.
conventional.
kinesin.(Kin-1).
with.
C-terminal.
truncation
Glide;.docked.kinesin.
motors;.
microlithographic.
rectifying.arrow.
designs
Inverted.motility.assay;.
kinesin-MT;.use.of.
microlithographic.designs.
to.maintain.MT.orientation;.
chemical.patterning;.
topography
Controlled.and.reliable.
guidance.of.molecular.
shuttles;.integration.with.
inorganic.devices.for.
transport,.sorting,.etc.
Has.functioned.to.properly.
orient.microtubules.shuttled.in.
a.closed.loop;.similar.concept.
in.Lin.et.al..(2008).successfully.
used.to.guide.shuttles.to.
sensor
Hiratsuka.et.al..
(2001);
Lin.et.al..(2008)
