Biomedical Engineering Reference
In-Depth Information
O
N
H
N
H
N
N
H
O
H
N
N
2
H
RPR-120535
O
H
H
N
N
2
N
N
N
H
O
N
H
2
RPR-126096
O
H
N
H
N
N
2
N
N
H
O
N
H
RPR120528
2
H
N
N
H
2
2
O
H
N
N
N
H
N
N
N
2
H
O
RPR120525
Figure 1.
Geometrically varied lipopolyamines for structure-activity
relationship studies.
on HeLa
cells. Results obtained in Table 1.1 show that although all the
lipopolyamines displayed high level of transfection, the “linear” RPR-
120535 showed the highest level of luciferase expression, followed
by the “branched” RPR-120525 and “« T » shaped” RPR-126096,
and finally the “globular” lipopolyamine RPR-120528.
The obtained lipopolyamines were assayed
in vitro
Table 1.1
Impact of polyamine geometry on expression of luciferase
on HeLa cell line in the presence or absence of serum. Results
are in RLU/10s./2 × 10
3
treated cells
N° RPR
Geometry
HeLa
120535
Linear
2.4 × 10
6
126096
« T » shape
5.5 × 10
5
120528
Globular
1.3 × 10
5
120525
Branched
5.8 × 10
5
In agreement with previous works [26], the geometry of
the cationic entity affects the transfection efficiency of the self-
assemblies with DNA.
The introduction of a variety of linkers between the lipid and
the polyamine did not affect substantially the transfection efficiency
Search WWH ::
Custom Search