Biomedical Engineering Reference
In-Depth Information
was no signiicant difference between both surfaces after 1 day of
incubation. The number of MG-63 cells on glass, np20, np80 and
m-HA was 357 ± 34, 299 ± 24, 215 ± 21, and 135 ± 13 mm
-2
after
5 day. These are consistent with the results of AO staining, which
showed that the cell coverage of np20 ilm was higher than that of
np80 and m-HA ilms after 5 days of culture (Fig. 12.7B).
Figure 12.7
(A) Cell density of MG-63 on different substrates for 1,
3, and 5 days.
n
= 6; *signiicantly different from m-HA,
p < 0.05. (B) Fluorescence microscopic photographs of MG-
63 cells (cultured for 5 day) on (a) glass, (b) np20, (c) np80,
and (d) m-HA [115]. See also Color Insert.
This study suggested that nano-HA particles could stimulate
osteoblastic proliferation as compared with m-HA. In addition,
this study further revealed that the cell proliferation was inversely
related to the HA particle sizes. Np20 was the most effective of the
three HA samples. This may be ascribed to the enhanced interfacial
adhesion of HA nanoparticles to cells, as well as the improved
penetration abilities of smaller HA's. Nano-HA's can provide a higher
