Biomedical Engineering Reference
In-Depth Information
proliferation and differentiation [116, 117]. Research has shown
that the incorporation of transforming growth factor-beta 1 results
in enhanced cellular function [118]. Thus, this route appears to offer
the potential for chemical and topographical control of cell behavior
and also drug delivery.
Figure 12.6
Osteoblast cells on a glass substrate coating with nanoscale
HA deposited via electrohydrodynamic atomization [18].
12.3.4
Size Effect of Hydroxyapatite Nanoparticles on
Proliferation and Apoptosis of Osteoblast Cells
Nano-hydroxyapatite (nano-HA), as a better candidate for an apatite
substitute of bone in biomedical applications than microsized
hydroxyapatite (m-HA), is suggested by many researches [115].
Nano-HA particles with diameters of ~20 nm (np20) and
~80 nm (np80) were synthesized and characterized. The size effects
of these nano-HA's and m-HA were studied on human osteoblast-
like MG-63 cells
in vitro
. These results demonstrate that both cell
proliferation and cell apoptosis are related to the size of the HA
particles. Np20 has the best effect on promotion of cell growth and
inhibition of cell apoptosis. This work provides an interesting view of
the role of nano-HA's as ideal biomedical materials in future clinical
applications.
As shown in Fig. 12.7A, the MG-63 cells on np20, np80, and
m-HA ilms proliferated with increasing culturing time (up to 5
days). Furthermore, cell number was signiicantly higher on np20
compared to m-HA after 3 and 5 days of incubation, though there
