Biomimetic ECM Scaffolds Prepared from Cultured Cells - Biomimetics: Advancing Nanobiomaterials and Tissue Engineering - page 243

Table 10.1. Main compositional molecules in ECM scaffolds prepared
from MSCs, chondrocytes and fi broblasts

ECM-M ECM-C ECM-F

Type I collagen +++++ +++++ +++++

Type II collagen

× × ×

Type III collagen + + ++

Fibronectin ++++ ++++ ++++

Vitronectin +++ +++ +

Laminin

+ + ++

Aggrecan + ++ +

Versican × + ×

Decorin + + +

“ + ” indicates detected and “ × ” indicates not detected

10.3 Autologous ECM Scaffolds

Autologous ECM (aECM) scaffolds should be a safe and reliable biomate-
rial candidate [33]. The development of aECM scaffolds has been strongly
anticipated for use in tissue engineering and regenerative medicine [34,
35]. Use of both autologous cells and autologous scaffolds should elimi-
nate negative host responses and lead to optimal tissue regeneration.
However, the availability of autologous sources of donor tissues and
organs is highly limited. It has been almost impossible to use such acel-
lular autologous matrices for tissue engineering. The ECM secreted by
autologous cells should be a potential alternate to acellular autologous
tissues and organs because some autologous cells can be expanded in vitro
and maintained under a pathogen-free condition.
The above described template method has been used to prepare mouse
aECM scaffolds by using mouse fi broblasts. Mouse fi broblasts (mF) are iso-
lated from the biopsies of 6-week-old ICR (Clrj:CD1) mice and expanded
in vitro . P2 mouse fi broblasts are seeded and cultured in the PGLA mesh
template for 10 days. Mouse autologous ECM (aECM-mF) scaffolds are
obtained after decellularization and removal of PLGA mesh template. The
aECM-mF scaffolds show mesh-like three-dimensional structures and the
primary composition is collagen I, collagen III, fi bronectin, vitronectin,
laminin, decorin and biglycan.