RECENT ADVANCES IN FLOW CYTOMETRY: PLATFORMS, TOOLS, AND CHALLENGES FOR DATA ANALYSIS - Flow Cytometry in Drug Discovery and Development - page 24

TABLE 2.1 Examples of Current Flow Cytometry Platforms and Key Features

Maximum Detection
Channels (Fluors/Scatter) Key Features

Main Application
Area Supplier Instrument Main Purpose Excitation Sources

Accuri
Cytometers, Inc.

C6 Flow
Cytometer

Cell analyzer 2 (488, 640 nm diode

lasers)

4 þ FSC þ SSC Benchtop; 6 log dynamic

range; low-pressure
fluidics

Basic and clinical
research

Amnis Corp. ImageStreamX Cell analyzer Up to 5 (405, 488, 560,

592, 658 nm)

10 þ bright field þ dark
field (with second
camera)

Imaging cytometer; dots are
also images;
phenotype þ morphology

Basic and clinical
research

Axetris Division,
Leister Process
Technologies

Axetris Impedance
Flow
Cytometer

Cell analyzer None, impedance

measurement

Cell size; membrane ca-
pacitance; cytoplasmic
conductivity

Biomarker independent;
microfluidic chip system;
reduced sample processing

Industrial and basic
research

BD Influx

BD Biosciences Cell sorter Up to 5 (from 405,

457, 488, 532, 561,
640 nm)

3/4-plex PMT
modules per laser line

Fully aseptic sorting;
multiparameter sorting;
flexible platform

Clinical research

BD Biosciences BD FACSAria II Cell sorter Up to 4 (from 375,

405, 488, 633 nm)

13 þ FSC þ SSC Aseptic sorting; optimized for

stem cells; fixed
alignments

Basic and clinical
research

BD Biosciences BD FACSCanto Cell analyzer Up to 3 (from 405,

488, 633 nm)

8 FSC SSC Approved for CIVD; carousel

and HTS option; fixed
alignment

Clinical use

þ þ

II

BD Biosciences BD FACSCount Cell analyzer 1 2 FSC SSC Approved for CIVD;

dedicated for HIV/AIDS;
minimal user intervention

Clinical use
(HIV/AIDS)

þ þ

Instrumenta-
tion System

BD Biosciences BD LSR II Cell analyzer 4-7 (special order,

from 11
wavelengths)

18 þ FSC þ SSC Fully flexible platform;

upgradeable; optimizes
excitation/emission

Basic and clinical
research

BD Biosciences BD

LSRFortessa

Cell analyzer 5 (from 11

wavelengths)

18 þ FSC þ SSC Reduced footprint (cf. LSR

II); upgradeable; optimizes
excitation/emission

Basic and clinical
research

(Continued )