Biomedical Engineering Reference
In-Depth Information
Factor 1
A: Cell
number
Factor 2
B: Type of
Fluorochrome
Factor 3
C: [Drug-fluorochrome]
(ug/mL)
Response 1
GMFI
Response 2
S/N ratio
0.5
10
6
FITC
1.0
95
10
1.0
10
6
FITC
1.0
62
6
0.5
10
6
Alexa 488
1.0
169
9
1.0
10
6
Alexa 488
1.0
100
10
0.5
10
6
Alexa 647
1.0
616
8
1.0
10
6
Alexa 647
1.0
339
7
0.5
10
6
FITC
2.5
199
20
10
6
1.0
FITC
2.5
107
10
0.5
10
6
Alexa 488
2.5
366
20
1.0
10
6
Alexa 488
2.5
159
16
0.5
10
6
Alexa 647
2.5
1404
18
10
6
1.0
Alexa 647
2.5
675
14
0.5
10
6
FITC
5.0
333
33
1.0
10
6
FITC
5.0
196
18
0.5
10
6
Alexa 488
5.0
559
31
10
6
1.0
Alexa 488
5.0
320
32
0.5
10
6
Alexa 647
5.0
2200
28
1.0
10
6
Alexa 647
5.0
1210
26
One factor
(a)
1500
Design-Expert® Software
original scale
GMFI
Design points
X1 = B: Type of fluorescent dye
1162.5
Actual factors
A: Cell concentration = 0.5e6
C: Concentration of labeled
detector = 2.5
825
487.5
2
150
FITC
Alexa 488
Alexa 647
B: Type of fluorescent dye
FIGURE 10.4
Solutions are tabulated from DoE with category factor and response levels.
Plots show the relationship between maximum signal and cell number (a) or type of fluorescent
dye (b) or cell number and signal/noise ratio (c). The experimental conditions selected from this
experiment are shown in bold text in the table.
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