Chemistry Reference
In-Depth Information
Table 18.2
Studies providing evidence for inhibition of nucleotide and base excision repair by arsenicals
Arsenic compound (lowest
effective dose)
Species (test system)
End Point
Pathway
Ref.
As
2
O
3
(1 mg/l)
Arsenate (6 mg/l)
As
2
O
3
(0.5 mg/l)
Arsenate (5 mg/l)
h. fi broblast (SF24, XP6KO,
XP2SA)
V79 cells
NER
47
removal of UV-induced photolesions
↓
comutagenicity with UV
Arsenite (20
µ
M)
CHO-K1 cells
removal of UV-induced photolesions
↓
NER
111, 112
coclastogenicity with UV
Arsenite (80
µ
M)
h. lymphocytes
SV40-transformed fi broblasts
(MRC5CV1)
Removal of B[
a
]P-induced lesions
↓
NER
117
Arsenite (100
µ
M)
Arsenite (2.5
µ
M)
h. fi broblast
Removal of UV-induced photolesions
↓
NER
113
Arsenite (1
µ
M)
CHO cells
h. fi broblast
Removal of UV-induced photolesions
↓
NER
86
Arsenite (10 mg/kg)
Sprague-Dawley rats
Removal of B[
a
]P-induced DNA adducts
NER
114
↓
Arsenite (1
M)
h. TK6 lymphoblastoid cells
removal of UV-induced photolesions
NER
115
µ
↓
comutagenicity with UV
Arsenite (5
M)
h. A549 adenocarcinoma lung
cell line
removal of BPDE-induced DNA adducts
NER
42
µ
↓
MMA
III
(2.5
M)
µ
DMA
III
(2.5
M)
µ
MMA
V
(250
M)
µ
DMA
V
(250
M)
µ
Arsenite (5
µ
M)
h. CRL2522 fi broblasts
h. colon HCT116 cancer cells
removal of BPDE-induced DNA adducts
↓
NER
116
MMA
III
(1
µ
M)
impairment of p53 induction in response
to BPDE treatment
DMA
III
(1
µ
M)
Arsenite (500
µ
M)
XPA
binding of XPA to the MMC C-DNA
interstrand crosslink
NER
122
↓
Arsenite (100
µ
M)
XPAfz
(peptide representing the XPA zinc
fi nger domain)
release of zinc from XPAzf
NER
42, 120
MMA
III
(10
µ
M)
DMA
III
(10
M)
MMA
V
(5 mM)
DMA
V
(no effect up to 10 mM)
µ
