Biology Reference
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Fig. 10. Clustering of T-invariants with trivial and small T-invariants shown in the bottom of the tree. The two main clusters are
colored in blue and red. Latin enumeration of clusters refers to the output as obtained from T
INA
N
ET
[Thormann
et al.
, 2009]
using the Tanimoto distance measure and 80% similarity cutoff. Roman enumeration labels clusters that form larger groups.
(Colours are visible in the online version of the article at
www.iospress.nl
.)
constitutes the influx and efflux of ASF/SF2 by
t140.ASF SF2 out
and
t103.ASF SF2 out
respectively.
However, the T-Invariants, which contribute to MCTS 5 (Tab. 2) are more complex and describe partially
the recognition of the 5'ss aided by the splicing factor ASF/SF2. They differ in the mode how other
splicing auxiliary factors like SC35, SF1, U2AF or FBP11, are involved in 5'ss recognition. Thus, all
T-Invariants missing
t103.ASF SF2 out
have in common that they support the binding of U1 snRNP. It
is known that E-complex assembly can heavily rely on the presence of SR proteins (auxiliar splicing
factors), which organize the cross-talk between 5'ss, branch point and 3'ss [Shen and Green, 2004]. In
such spliceosomes a critical level of SR proteins like ASF/SF2 or SC35 should be beneficial and path-
