Surface-Enhanced Raman Scattering-Based Bioimaging - Nanotechnology for Biomedical Imaging and Diagnostics - page 329

(a)

SH

HS

HAuCl 4 .4H 2 O
Ascorbic acid

Au core Au/BDT core

Bright

PEG-ERbB2-BRIGHT

(c)

(b) Targetting

SH-PEG-ERbB2

Nucleus

Bright

2µm

(e)

(d)

10 µM 10 µM

(g)

(f)

1 4

1

4

5

5

2

2

3

10 µM 10 µM

3

figure 11.6 (a) Synthesis of BRigHTs involves the exchange of gold nanoparticle sur-
factant with a Raman reporter. (b) Schematic diagram illustrating the biofunctionalization in
vitro targeting of BRigHTs to SKBR3 breast cancer cells. (c) SEM image of BRigHT-targeted
fixed SKBR3 cell reveals that the nanostructures largely remain as individual particles. (d) a
bright-field optical micrograph of BRigHT-targeted SKBR3 cells and (e) corresponding
dark-field micrograph showing the intense Rayleigh scattering from BRigHTs. intensity map
of 1078 cm −1 Raman band from BRigHTs targeted to SKBR3 cell collected using (f) 785 nm
and (g) 632.8 nm as excitation sources, respectively. (Reprinted with permission from Ref.
[61]. © Wiley.) ( See insert for color representation of the figure.)