FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 251

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

1263 cm −1 T, A (ring breathing modes of the

DNA/RNA bases); = C-H bend
(protein)

* 12

1264 cm −1 Triglycerides (fatty acids) * 49

Ring stretch * 14

δ(= CH) of lipids * 17

1265 cm −1 PO 2 − asymmetric (phosphate I) * 48

CH α' rocking * 6

Amide III of collagen * 41

Amide III (collagen assignment) * 19

Amide III * 33, 35

ν(CN),δ(NH) amide III, α-helix,
collagen (protein assignment)

* 33

Phenylalanine stretching
(C-C6H5) mode

* 35

1266 cm −1 Amide III (of proteins in the

α-helix conformation)

* 3, 20

ν(CN), δ(NH) amide III, α-helix,
collagen, tryptophan (protein
assignment)

* 20

δ (= C-H) cis * 7

Amide III (α-helix) * 43

1267 cm −1 C-H (lipid in normal tissue) * 54

Amide III (collagen assignment)

1268 cm −1 δ (= C-H) (phospholipids) * 43

Ring stretch * 14

1268/9 cm −1 Amide III (collagen assignment) * 19

1269 cm −1 Structural proteins like collagen * 32

Cytosine (ring breathing modes
of the DNA/RNA bases)

* 4

1270 cm −1 Typical phospholipids * 54

Amide III band in proteins * 89, 90

Has traditionally been attributed
to Amide III, a C-N stretch from
alpha helix proteins

* 91

C= C groups in unsaturated fatty
acids

* 2

1272/3 cm −1 CH α' rocking * * 6

1275 cm −1 Amide III * 7

1276 cm −1 N-H thymine * 48

( Continued )