FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 250

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

1244/5 cm −1 PO 2 − asymmetric (phosphate I) * 48

1245 cm −1 PO 2 − asymmetric * 52, 78

Amide III * 7

1246 cm −1 Amide III (of collagen) * 3

PO 2 − asymmetric * 38, 78

1247 cm −1 PO 2 − asymmetric (phosphate I) * 48

Amide III (collagen assignment) * 19

1247/8 cm −1 Guanine, cytosine (NH 2 ) * 21

1248 cm −1 PO 2 − asymmetric * 78

1250 cm −1 Amide III * * 22

1250/2 cm −1 Guanine, cytosine (NH 2 ) * 21

1252 cm −1 C-O 4 aromatic stretch * 6

1254 cm −1 Formalin contamination on fixed

tissues

* 41

C-N in-plane stretching * 28

1255 cm −1 Lipids * 24

Amide II (?) * 50

1256 cm −1 PO 2 − asymmetric (phosphate I) * 48

1257 cm −1 A, T (ring breathing modes of the

DNA/RNA bases); Amide III
(protein)

* 12

Ring stretch * 14

Ring C-C stretch * 14

1258 cm −1 Amide III, adenine, cytosine * 9

Protein peak * 17

Amide III, β-sheet/adenine and
cytosine in tumours

* 17

Amide III (β-sheet), adenine,
cytosine

* 16

1259 cm −1 Guanine, cytosine (NH 2 ) * 21

Amide III * 19

1260 cm −1 Amide III (protein band) * 24

Protein band * 25

Second amide (?) * 45

Amide III (unordered) * 9

Structural protein modes of
tumours

* 26

Amide III vibration mode of
structural proteins

* 49

1262 cm −1 PO 2 − asymmetric (phosphate I) * 48