FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 249

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

Asymmetric phosphate [PO 2 −
(asym.)] stretching modes
(Phosphate stretching modes
originate from the
phosphodiester groups of
nucleic acids and suggest an
increase in the nucleic acids in
the malignant tissues. Generally,
the PO 2 − groups of
phospholipids do not contribute
to these bands.)

* * 3

64

Phosphate stretching bands from
phosphodiester groups of
cellular nucleic acids

* 51

ν as Phosphate * 77

1242 cm −1 PO 2 − asymmetric * 78

Collagen I and IV * 78

Amide III * 40, 50

Amide III collagen * 51

Amide III (β-sheet and random
coils)

* 43

1243 cm −1 Amide III * 24

* 77, 80

ν(PO 2 − ) asymmetric stretching of
phosphodiesters

Asymmetric phosphate [PO 2 −
(asym.)] stretching modes
(Phosphate stretching modes
originate from the
phosphodiester groups of
nucleic acids and suggest an
increase in nucleic acids in the
malignant tissues. Generally, the
PO 2 − groups of phospholipids
do not contribute to these
bands.)

* * 3

64

Phosphate in RNA
C-O 4 aromatic stretch

* 23

Amide III of collagen (CH 2
wagging, C-N stretching) and
pyrimidine bases (C, T)

*
*

6
9

1243/4 cm −1 Collagen I * 78

1244 cm −1 Collagen I and IV * 78

Asymmetric phosphate stretching
(ν as PO 2 − )

* 23, 38, 52

( Continued )