FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 237

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

Symmetric phosphate
[PO 2 − (sym)] stretching

* 40, 64

PO 2 − symmetric * 78

Stretching PO 2 − symmetric * 23

Absorbance by the
phosphodiester bonds of the
phosphate/sugar backbone of
nucleic acids

* 40

Nucleic acid region * 40

Nucleic acid-phosphate band * 72

C-N stretching of proteins and
lipids, ν(C-C) and ν(C-O) of

* 17

phospholipids

1084-6 cm −1 * 51

ν s (PO 2 − ) of nucleic acids

1085 cm −1 PO 2 − symmetric (phosphate II) * 48

PO 2 − symmetric * 78

Mainly from absorption bands of
the phosphodiester group of
nucleic acids and membrane
phospholipids, and partially
protein (amide III). The band
originating from sugar chains
(C-OH band) overlaps.

* 78

Mainly from phospholipid
phosphate and partly from
oligosaccharide C-OH bonds

* 56

Phosphate ester * 56

1086 cm −1 Symmetric phosphate stretching

modes or ν(PO 2 − ) sym.
(Phosphate stretching modes
originate from the
phosphodiester groups in
nucleic acids and suggest an

* 66

increase in the nucleic acids in the
malignant tissues.)
PO 2 − symmetric * 78

ν(PO 2 − ) symmetric stretching of
phosphodiesters

* 77, 80

ν(C-C) gauche * 43

1087 cm −1 PO 2 − symmetric (phosphate II) * 48

ν 1 CO 3 2− , ν 3 PO 4 3− , ν(C-C) skeletal
of acyl backbone in lipid ( gauche
conformation)

* 3

( Continued )