FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 236

Table 8.1 ( Continued )
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

Collagen and phosphodiester
groups of nucleic acids

* 63

1081 cm −1 ν 1 CO 3 2− , ν 3 PO 4 3− , ν(C-C) skeletal

of acyl backbone in lipid ( gauche
conformation)

* 3

Symmetric phosphate stretching
modes or ν(PO 2 − ) sym.
(Phosphate stretching modes
originate from the
phosphodiester groups in nucleic
acids and suggest an increase in
the nucleic acids in the
malignant tissues.)

* 66

* 77

ν(PO 2 − ) symmetric stretching of
phosphodiesters

Phosphate I in RNA * 23

One of the triad peaks of nucleic
acids (along with 1031 and
1060)

* 50

1082 cm −1 Carbohydrate residues of

collagen

* 24

Carbohydrates peak for solutions * 24

Nucleic acids * 24

PO 2 − symmetric * 78

Phosphate band * 79

Collagen * 40

Symmetric phosphate stretching
band of the normal cells

* 51

Carbohydrates * 30

Phospholipids O-P-O and C-C * 46

1083 cm −1 C-N stretching mode of proteins

(and lipid mode to a lesser
degree)

* 10

PO 2 − symmetric
C-N stretching mode of proteins
(and lipid mode to a lesser
degree)

* 78

ν s PO 2 − of phosphodiesters of
nucleic acids

* 9

* 61

1084 cm −1 Phosphodiester groups in nucleic

acids

* 24

DNA (band due to
PO 2 − vibrations)

71