FTIR and Raman Characteristic Peak Frequencies in Biological Studies - Vibrational Spectroscopy for Tissue Analysis - page 214

Table 8.1
Major Characteristic Peak Frequencies Reported in the Literature

Reference
Number Peak Assignment FT-IR Raman

200-70 cm −1 CH 3 torsion, usually not detected

experimentally and theoretically

* 1

250 cm −1 (CH 3 ) ester modes: deformations

torsion (with very low Raman
activity)

* 1

415 cm −1 Phosphatidylinositol * 2

418 cm −1 Cholesterol * 3

428 cm −1 Symmetric stretching vibration of

ν 2 PO 4 3− (phosphate of HA)

* 3

429 cm −1 Cholesterol, cholesterol ester * 2

436 cm −1 Thymine (ring breathing modes

of the DNA/RNA bases)

* 4

445 cm −1 N-C-S stretch (one of three

thiocyanate peaks, with 2095
and 735 cm −1 )

* 5

447/54 cm −1 Ring torsion of phenyl(2) * 6

472/5 cm −1 C α = C α , torsion and C-OH 3

torsion of methoxy group(4)

* 6

477 cm −1 Polysaccharides, amylose

Polysaccharides, amylopectin

*
*

7
7

481 cm −1 DNA * 8

484-90 cm −1 Glycogen * 9

490 cm −1 Glycogen * 10

505/8 cm −1 C-OH 3 torsion of methoxy

group(1)

* 6

509 cm −1 S-S disulfide stretching band of

collagen

* 3

ν(S-S) gauche-gauche-gauche
(amino acid cysteine)

* 7

519 cm −1 Phosphatidylinositol * 2

521 cm −1 C α = C α , torsion and ring torsion

of phenyl(1)

* 6

524 cm −1 S-S disulfide stretching in

proteins

* 9, 10

Phosphatidylserine * 2

ν(S-S) gauche-gauche-trans (amino
acid cysteine)

* 7

538 cm −1 Cholesterol ester * 2

Disulfide bridges in cysteine (this
peak seems to be the main factor
for the FT-Raman determination
of the boundaries between
healthy and pathological tissue)

* 11