Biomedical Engineering Reference
In-Depth Information
8
FTIR and Raman
Characteristic Peak Frequencies
in Biological l Studies
Introduction
This chapter describes some of the most widely used peak frequencies of the
two spectroscopic methods, Fourier transform infrared (FTIR) and Raman
spectroscopy, and their assignments. It is designed to prepare a database of
molecular fingerprints for defining the chemical compounds and introduc-
ing the most important characteristic peaks.
In spectroscopic studies, accurate peak definitions can have a great
influence on the reliability of the results. Combing through the literature,
it becomes apparent that most scientists have mainly used the previously
published articles to define the data acquired from the collected spectra.
However, without having a reliable and detailed database that can cover most
of the famous peaks in the spectral range, it would be a time-consuming task
to find the meaning of different peak intensities in the related articles. This
chapter tries to alleviate that shortcoming.
In spite of applying different methods, there seems to be a considerable
similarity in defining the peaks of identical spectral areas. As a result, it is
believed that preparing a unique collection of the frequencies encountered
in spectroscopic studies can lead to significant improvements in both the
quantity and quality of spectroscopic studies of biological molecules. As a
result, this chapter presents a precise database (Table 8.1) on the major char-
acteristic peak frequencies reported in the literature on studies of natural
tissues by spectroscopic means and will surely be of considerable assistance
to scientists focusing in this subject area.
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