Infrared and Raman Spectroscopy and Imaging of Breast Cancer - Vibrational Spectroscopy for Tissue Analysis - page 122

0.34
0.32
0.30
0.28
0.26
0.24
0.22
0.20
0.18

Black : LNG
Blue : ING
Red : HNG

0.16
0.14
0.12
0.10
0.08
0.06
0.04

3500 3000 2500

Wavenumbers (cm -1 )

2000 1500 1000

Figure 5.11 (See colour insert.)
FTIR spectra of different DCIS grades.

Table 5.3
Peak Assignments of the FTIR Spectra of Normal Breast Tissue and Breast Carcinoma

Approximate Peak Intensity Assignment Ref. No

3290 cm −1 N-H asymmetric stretching modes in

proteins and nucleic acids

21,31

3085 cm −1 N-H symmetric stretching modes in

proteins and nucleic acids

21,31

2960 cm −1 ν as CH 3 of lipids, DNA, and proteins 20

2920 cm −1 ν as CH 2 31,36,37

2850 cm −1 31,36,37

ν s CH 2

1630 cm −1 Amide I (in-plane stretching of the C=O

bond of the peptide backbone, weakly
coupled to stretching of the C-N and
in-plane bending of the N-H bond)

20,41,42

1540 cm −1 Amide II (primarily N-H bending coupled

to C-N stretching, or C-N stretching and
C-N-H bending vibrations)

20,29,37,41,42

1452 cm −1 Overlapping asymmetric CH 3 bending &

CH 2 scissoring (is associated with elastin,
collagen, and phospholipids

28,43

1372 cm −1 CH 3 (Symmetric CH 3 bending) 37,42

1310 cm −1 Amide III, Symmetric stretch 44,45,46

1260 cm −1 Amide III, ν(CN), δ(NH) 44,45,46

1216 cm −1 C-O (C-OH groups of serine, threosine and

tyrosine of proteins)

46,47

1080 cm −1 O-P-O (Symmetric stretching of phosphate

groups of phosphodiester linkages)

29,42

1030 cm −1 O-P-O (Phosphate groups of phosphorylated

proteins & cellular nucleic acids

41