Biomedical Engineering Reference
In-Depth Information
Table 2 Summary of applications of dielectric spectroscopy to microbial cell cultures, with details of the device and settings used, the data processing
applied and the cell density range measured
Device
Application
Settings and data pre-processing
Cell
density
References
Four-terminal dielectric spectrometer,
Aber biomass monitor
S. cerevisiae
Low-pass filter with time constant of 1 s,
measurements at 0.4 MHz
[
49
-
51
]
Aber instrument 214 A
Baker's yeast
25 frequencies from 0.2 to 10 MHz;
ANN compared with PLS and PCA
0-70 g/L
[
44
]
Aber biomass monitor 386SX IBM PC
with DT2911-PGH 12-bit A/D I/O
board in combination with
PERMSTAT.exe, an
in-house built QUICKBasicv4.5
program
Baker's yeast
0.4 MHz and 9.5 MHz were used during the
fermentation; offline, each sample was scanned
at 12 frequencies from 0.2 mHz to 9.6 MHz;
various non-linear times series
-
[
45
]
BM 214-M
Baker's yeast
Study of data treatment and filtering
(Butterworth etc.)
12-25 g/
L
[
46
]
Three-electrode Aber instrument probes
BM 214 A
Lysed baker's yeast
0.4 and 10 MHz
-
[
47
,
48
]
Aber BM 214A
Candida utilis, V. paradoxus
DSM 4065
0.6 and 10 MHz;
moving-point average
0-2 g/L
[
52
,
53
]
Aber BM 214
Streptomyces claviguleris
Dual frequency 0.1-1.0 MHz and approximately
10 MHz, low-pass filter to 1 s and low-pass
range, low and high frequencies chosen as 0.4
and 10 MHz, data acquisition at 0.2 per min
0-30 g/L
[
54
]
Aber 214 M
Streptomyces genus
Dual frequency 0.1-1.0 MHZ and 9.6 MHz, low
range, low-pass filter to 1 s, excitation
frequency 0.4 MHz, data acquisition at 0.2 per
min, working volume of the reactor of 200 L;
Raw conductance measurements averaged over
25 points
-
[
55
]
(continued)
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