Biomedical Engineering Reference
In-Depth Information
Table 2.13.
Calibration kits for polyacrylamide gel electrophoresis (M
r
in
kD)
Peptide marker kit
M
r
LMW kit
M
r
mg/ml
Myoglobin peptides
16.949
14.404
10.7
8.159
6.214
2.512
Phosphorylase b
96
0.67
Serum albumin
67
0.83
Ovalbumin
43
1.47
Carbonic acid anhydrase
30
0.83
Trypsin inhibitor
20
0.80
α
-Lactoglobulin
14
1.21
HMW Native Kit
M
r
mg/ml
HMW SDS Kit
M
r
mg/ml
Thyroglobulin
669
0.76
Myosin
210
0.25
α
Ferritin
440
0.5
2
-Macroglobulin
170
1.00
β
Katalase
232
0.36
-Galactosidase
116
0.16
Lactate
140
0.48
Transferrin
90
0.17
dehydrogenase
Serum albumin
Glutamate
53
0.18
66
0.4
dehydrogenase
Manufacturer: GE Healthcare; formerly Amersham Biosciences
MW SDS-70
M
r
mg/ml
MW SDS-200
M
r
mg/ml
Serum albumin
67
1.5
Carbonic acid
30
1.0
Ovalbumin
43
1.5
anhydrase
Ovalbumin
Pepsin
35
5.0
43
1.5
Tr y p s i n o g e n
2 4
2 . 0
Serum albumin
67
1.5
α
-Lactoglobulin
17
1.0
Phosphorylase b
96
1.0
β
Lys ozy m
14
1.0
-Galactosidase
116
1.0
Myosin
204
1.7
Manufacturer: Sigma Aldrich
SDS-PAGE Rainbow marker
Low
High
Color
M
r
M
r
Myosin
220
Blue
Phosphorylase b
97.4
Brown
Serum albumin
66
Red
Ovalbumin
46
46
Yellow
Carbonic acid anhydrase
30
30
Orange
Trypsin inhibitor (soy bean)
22
21.5
Green
Lysozym
14.3
14.3
Purple
Aprotinin
6.5
Dark blue
Insulin B chain
3.4
Blue
Insulin A chain
2.4
Blue
Manufacturer: BioRad
