Biomedical Engineering Reference
In-Depth Information
Other dyes for cathodic (acidic) electrophoresis systems are
Pyronin Y, 1-napthyl red and methyl red, the latter only for systems
with pH below 3.
2.3.2 Marker Proteins for the Polyacrylamide Gel
Electrophoresis
Prerequisites for the use of a protein or polypeptide as calibrator
in PAGE are the accessibility in a pure, definite state, its known
Table 2.12.
Marker proteins for polyacrylamide gel electrophoresis
Protein
M
r
in kD
With SDS
Without SDS
Glucagon
3.483
Insulin (pig)
5.733
Insulin A chain
2.533
Insulin B chain
3.495
Trypsin inhibitor (Aprotinin; bovine)
6.5
Cytochrome c (pig)
12.5
Lysozyme (hen egg)
14.3
α
-Lactalbumin (bovine)
14.4
Hemoglobin (bovine)
16
Myoglobin (horse)
16.95
Myoglobin. BrCN-fragment III
2.512
Myoglobin. BrCN-fragment II
6.214
Myoglobin. BrCN-Fragment I
8.159
β
-Lactoglobulin (bovine)
18.4
Trypsin inhibitor (soy bean)
20.1
Trypsinogen (bovine)
24
Chymotrypsinogen A (bovine)
25.7
Carbonic acid anhydrase (bovine)
29
Pepsin (pig)
34.7
Aldolase (rabbit)
40
Ovalbumin (hen egg)
42.7
Glutamate dehydrogenase (bovine)
53
Pyruvate kinase (rabbit)
57.2
Serumalbumin (bovine)
66.25
Transferrin (human)
76
Ovotransferrin (hen egg)
76 - 78
Phosphorylase b (rabbit)
94
β
-Galactosidase (
E.coli
)
116.3
Lactate dehydrogenase
36
140
α
-Macroglobulin (bovine)
170
Myosin (rabbit)
205 - 215
Catalase
57.5
232
Thyroglobulin
305
Ferritin (horse)
18.5 + 220
440
