Combining Digital Holographic Microscopy with Microfluidics: A New Tool in Biotechnology - Biomedical Optical Phase Microscopy and Nanoscopy - page 200

a trivial stroboscopic illumination, depending also on the velocity fields of the particles, the
influence of the light on the paths can be reduced to become totally negligible, making this
method a full noninvasive 3D tracking approach.
10.4 Conclusions
Twin laser beams, passing through the same microscope objective, allow simple and
compact digital holographic microscope in off-axis configuration. The effectiveness of this
configuration has been proved. It is particularly suitable for phase contrast analysis and 3D
imaging of biological samples in a microfluidic environment. We experimentally
implemented the setup, and demonstrated the ability to drive micro-objects or track them in
the sample volume. An advantage of this arrangement is to measure the phase contrast
signature of the above-mentioned particle by digital holographic recording. For tracking
experiments, we validated the model either numerically or from the experimental point of
view. We demonstrated the 3D tracking and quantitative phase contrast microscopic
analysis on multiple cells that were moving in a microfluidic environment. The optical
configuration is very simple as it is made by only two beams. The beams could be also
produced by various configurations such as diffraction grating at the input pupil of the
microscope objective or even by using a spatial light modulator.
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