Biomedical Engineering Reference
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Figure 6.11
Label-free dynamic monitoring of cell death processes. Time-dependent observation of living
pancreatic tumor cells (PaTu 8988 T) after addition of taxol. First row: representative gray level
coded unwrapped phase distributions after toxin addition; second row: representative phase
contrast images from a control measurement without taxol addition. Source: Modified from
Ref.
[78]
.
6.6 Quantitative Cell Division Monitoring
In Refs.
[79
81]
, it was shown that quantitative phase imaging can be used for the
observation of cellular growth by the determination of changes in the density of the
intracellular content. Here, the use of DHM for label-free quantitative dynamic monitoring
of endothelial cell division
[82]
by cell tracking and cell thickness monitoring is
demonstrated. For the experiments, HBMECs (see
Section 6.5
) were cultivated in a Petri
dish. The cells were observed with an iMIC with attached DHM module as shown in
Figure 6.1
. An incubator was used for a stabilized temperature of 37
C.
Figure 6.12
shows representative results for the obtained quantitative DHM phase contrast
images (coded to 256 gray levels). After
t
5
19.7, 32.5, and 37.7 h the HBMECs denoted as
A, B, and D, respectively, underwent a cell division. The corresponding daughter cells after
the cell division process are designated as a
1
,a
2
,b
1
,b
2
, and d
1
,d
2
. Cell C did not divide
during the experimental period. It was observed that the phase contrast increases
significantly before mitosis. During the cell cycle phases in which the cells adhere on the
substrate subcellular regions with a higher density like the nucleus, the nuclear membrane
and the nucleoli become visible. In analogy, during metaphase, anaphase, and telophase,
bright areas in the phase contrast images indicate the separation of the chromosomes, in
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