Biomedical Engineering Reference
In-Depth Information
HBMEC
EA.hy 926 cell
14 µm
(A)
0µm
t
= 0 h
t
= 35 h
t
= 51 h
t
= 0 h
t
= 6.3 h
t
= 20.8 h
8
8
(B)
15
15
t
= 0 h
t
= 0 h
t
= 35 h
t
= 6.3 h
6
6
t
= 51 h
t
= 20.8 h
10
10
4
4
5
5
2
2
0
0
0
0
0
10
20
30
40
0
10
20
30
40
x
(µm)
x
(µm)
(C)
8
8
15
15
6
6
10
10
4
4
5
5
2
2
0
0
0
0
0 0 0 0 0
50
0 0 0
30
40
0
t
(h)
t
(h)
Figure 6.9
DHM investigation of Stx1-induced cell death of a single HBMEC and an EA.hy cell. (A) False
color-coded digital holographic phase contrast images of cells at indicated time after Stx1
addition. The magnification bars correspond to 10
m; (B) cross-sections through the
quantitative DHM phase images whereby the x-axis matches the lines in panel (A). The parameters
Δϕ
μ
and d denote phase contrast in radian and corresponding cell thickness; (C) temporal
dependence of the maximum OPL
Δϕ
max
and the corresponding maximum cell thickness d
max
.
Source: Modified from Ref.
[75]
.
(
n
medium
5
1.337) that was determined on suspended cells as described in
Section 6.4
.
Figure 6.9C
shows the temporal dependence of the maximum OPL
Δϕ
max
and the
corresponding maximum cell thickness
d
max
.
For the HBMEC (
Figure 6.9
, left panels), the first observed reaction was cell rounding after
20 h followed by an increase in the cell thickness by up to 50% after 35 h (
Figure 6.9C
, left
panel), with simultaneously decreased intracellular fluctuations. After 45 h, the phase
contrast started to decrease as a sign of progressive cell disintegration. Finally, the cell
death, accompanied by an extensive cell leakage, occurred after
,
51 h of Stx1 treatment
(
Figure 6.9A
, left panel). In contrast, initial Stx1-mediated cell rounding of the EA.hy 926
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